An explant outgrowth culture system for airway ciliated cells
- VernacularTitle:呼吸道纤毛上皮细胞的组织块法培养
- Author:
Kuiji WANG
;
Luo ZHANG
;
Demin HAN
;
Hong WANG
;
Erzhong FAN
;
Xiaohong SONG
;
Zhongyan LIU
- Publication Type:Journal Article
- Keywords:
Cilia;
Epithelial Cells;
Cell Culture;
Mucins
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To develop an explant outgrowth culture system for airway ciliated cells and explore the usefulness of the system.METHODS Mucosal specimens from rabbit tracheal epithelium were seeded into collagen-coated cover-slips. After 7 days,the specimens were characterized by Photo- contrast microscopy,transmission and Scanning electron microscope. They were also examined with hematoxylin-eosin staining and mucin-5AC immunohistochemistry. Ciliary beat frequency was measured with high-speed digital microscopy. RESULTS Many ciliated cells with good viability and goblet cells were found in the culture system. Immunohistochemistry analysis revealed that mucin- 5AC antibody labeled mainly in the goblet cells.The ciliated cells were differentiated well detected by electron microscope. At (30?1)℃,the basal ciliary beat frequency was (13.2?0.9) Hz. CONCLUSION The method may be useful in establishing a culture system for ciliated cells and the system will be suitable for the long-term studies of mucociliary transport system.
