The effect of co-transfection of p53 and angiostatin gene in SG7901
- VernacularTitle:联合转染p53与血管生成抑素基因对人胃癌细胞系SG7901生长的影响
- Author:
Xiangjin CHEN
;
Yueyong ZHU
;
Zhenting HU
;
Huihao ZHANG
;
Dongpo XU
;
Mingren LI
- Publication Type:Journal Article
- Keywords:
p53;
Angiostatin;
Gene therapy;
Carcinoma
- From:
Chinese Journal of Postgraduates of Medicine
2006;0(36):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the co-transfection of p53 and angiostatin gene in the inhibition of SG7901. Methods Transfected the pVITRO2-hp53-hAS into SG7901 with lipofectamine.After transfection, RT-PCR were used to know whether the aimed gene had been transfected and expressed or not. Cell clones trial, MTT growth curve, cell cycle measuring were used to analyze the differences. Results The cells were suppressed by the two genes and inhibition of the combined genes is more powerful than single one. Conclusion The effection of combined genes pVITRO2-hp53-hAS on SG7901 is stronger than either single one. Combined-gene-therapy is a useful anti-carcinoma method.
