MDR1 specific ribozyme reverses multidrug resistance (MDR) of human hepatocellular carcinoma, an in vivo experimental study
- VernacularTitle:MDR1特异性核酶逆转肝癌多药耐药的实验研究
- Author:
Yongshun GAO
;
Xiaoping CHEN
;
Shuijun ZHANG
;
Lei DING
;
Kaiyan LI
;
Zaide WU
- Publication Type:Journal Article
- Keywords:
Carcinoma, hepatocellular;
Multidrug resistance-associated proteins;
Ribozyme;
Nude mice
- From:
Chinese Journal of General Surgery
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the reversal of MDR by using anti-MDR1 ribozyme N2A + tRNAimet-iMDR1- sRz (sRz) in nude mice bearing human hepatocellular carcinoma. Methods The nude mice model with implanted human hepatocellular carcinoma was randomly devided into group A ( saline 40?l + Lipofect AMINE?000 10?l), B( N2A + tRNAimet10?g/40?l + Lipofect AMINE?000 10?l) and C(sRz 10?g/40?l + Lipofect AMINE?000 10?l). After one week, mice was peritoneally injected E-ADM 15 mg?kg-1 once a week for 4 weeks. The size of tumors was measured with B-ultrasound and the tumor inhibition rate was calculated. One week after chemotherapy mice was sacrificed and MDR1 mRNA and P-gp were investigated with RT-PCR and Western blot. Results In group C tumor shrank upon each chemotherapy (F = 659. 99, P
