Effect of silencing hyaluronidase gene HYAL1 by RNA interference on invasive potential of human breast cancer cell lines
- VernacularTitle:siRNA沉默HYAL1基因表达对人乳腺癌细胞侵袭力的影响
- Author:
Jinxiang TAN
;
Xiaoyi WANG
;
Guosheng REN
;
Gang TU
;
Xiaotao LI
;
Liang RAN
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
RNA interference;
Hyaluronoglucosaminidase;
Neoplasm invasiveness
- From:
Chinese Journal of General Surgery
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of RNA interference (RNAi) on HYAL1 gene mRNA expression and the invasive potential of human breast cancer cell lines. Methods Chemically synthesized double stranded RNA (dsRNA) targeting HYAL1 was transfected into human breast cancer cell lines MDA-MB-231, MDA-MB453S, ZR-75 and ZR-75-30 using SiPORT Lipid. The transfection efficiency was observed under fluorescence confocal microscopy. Expression of HYAL1 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). Cell penetrate matrigel capacity were determined by in vitro experiment. Results HYAL1 -siRNA effectively inhibited HYAL1 mRNA expression ( P
