The expression of mammalian target of rapamycin in autogenous vein graft in rats
- VernacularTitle:mTOR信号转导通路在自体移植静脉中的表达及意义
- Author:
Xinhua HU
;
Jun YANG
;
Chengwei LIU
;
Zhishen ZHANG
;
Qiang ZHANG
- Publication Type:Journal Article
- Keywords:
Transplantation;
Gene expression;
Veins
- From:
Chinese Journal of General Surgery
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of mammalian target of rapamycin (mTOR) in autogenous vein graft. Methods Autogenous vein graft model was established in 64 Wistar rats. Graft vein was harvested 6 hours, 1 day, 3 days, 1 week, 2 weeks, 4 weeks, 6 weeks and 8 weeks after transplantation. mTOR mRNA was measured by RT-PCR and in situ hybridization. Western blotting and immunohistochemistry methods were used to detect the expression of mTOR. PCNA expression was detected. Results The vein graft mRNA and protein expression of mTOR increased soon after transplantation. mTOR mRNA reached the peak 1 to 2 weeks after surgery [(48?18)% and (33?11)% respectively, P
