Effect of S-methylisothiourea on activities of superoxide dismutase and glutathione peroxidase in myocardial tissues of rats treated with adriamycin
- VernacularTitle:S-甲基异硫脲对阿霉素致大鼠心肌组织超氧化物歧化酶、谷胱甘肽过氧化物酶活性改变的影响
- Author:
Guanming YANG
;
Shengtao SUN
;
Shuquan LI
;
Weixiong LIN
;
Siyuan YE
;
Jilin LI
;
Shanxiu LIN
- Publication Type:Journal Article
- Keywords:
S-methylisothiourea;
Doxorubicin;
Nitric oxide synthase;
Nitric oxide;
Superoxide dismutase;
Glutathione peroxidase;
Gene expression
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of S-methylisothiourea(SMT) on adriamycin(ADM)-induced changes of superoxide dismutase and alutathione peroxidase activities in myocardial tissue of rats.METHODS: Rats were treated with ADM by intraperioneal injection (10.0 mg/kg body weight),and then the ADM-treated rats were intervened by SMT at different dosages by intravenous injection(once a day for three days).The contents of malondialdehyde(MDA) and NO_2~-/ NO_3~-,the activities of manganes superoxied dismutase(MnSOD),copper-zinc superoxide dismutase(Cu-Zn SOD),glutathione peroxidase(GPx) and inducible nitric oxide synthase(iNOS) were determined by spectrophotometry in myocardial tissue.The activity of isoenzymic CK-MB of creatine kinase(CK) was determined by enzymic rate method in serum.The expression of MnSOD mRNA,Cu-ZnSOD mRNA,GPx mRNA and iNOS mRNA were detected by reverse transcription-ploymerase chain reaction.RESULTS: The contents of MDA,NO~-_2/ NO~-_3,and iNOS activity of myocardial tissue,the activity of CK-MB in serum in SMT(5.0,10.0,20.0 mg/kg) intervention groups were significantly lower than those in ADM-treated group(P
