Analysis of gene structure and diversity of new alternative spliced variants of prostate specific membrane antigen
- VernacularTitle:前列腺特异膜抗原剪接变异体的基因结构和多态性分析
- Author:
Kaiyuan CAO
;
Na XIAO
;
Lin XU
;
Guangqing YUAN
;
Shuqin DAI
;
Xiaorong HUANG
;
Xiaodong TIAN
;
Shaopeng QIU
- Publication Type:Journal Article
- Keywords:
Prostate-specific antigen;
Splice variant;
Prostatic neoplasms
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To find out the gene structure and diversity of protate specific membrane antigen(PSMA) alternative spliced variants, and probe into the pathogenesis of prostate cancer.METHODS: 5'-RACE and 3'-RACE methods were used to amplify the 5' and 3'end of alternative spliced variant and then those viariants were sequenced for analyzing the gene stucture and diversity of PSMA alternative spliced variants of prostate cancer tissues.RESULTS: Four new alternative spliced variants of PSMA were discovered from prostate cancer tissues.Compared with reported PSMA alternative spliced variants,different insertions and deletions existed in different sites of those new variants.CONCLUSION: The discovery of the new variants confirms the diversity of PSMA spliced variants and provides the clues for seeking the target of diagnosis and therapy of prostate cancer.
