Determination of Glycyrrhetic Acid in Zhenkening Capsules by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定镇咳宁胶囊中甘草次酸的含量
- Author:
Jiancheng LIANG
;
Yikun HUANG
;
Sanping ZHANG
;
Zeyu LUO
;
Zhen CAI
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Zhenkening capsule;
Glycyrrhetic acid;
Content determination
- From:
China Pharmacy
2005;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the content of glycyrrhetic acid in zhenkening capsules by RP-HPLC.METHODS:The separation was performed on Hypersil C 18,the mobile phase consisted of methanol-water-glacial acetic acid(87∶13∶2.5)with flow rate at 1.0ml/min and detection wavelength at 254nm,the column temperature was set at room temperature.RE-SULTS:The calibration cure of glycyrrhetic acide was linear in the concentration range of 10～120?g/ml(r=0.9999),the average recovery was 99.68%(RSD=0.49%).CONCLUSION:This method is simple,fast,accurate,and suitable for the determination of glycyrrhetic acid in zhenkening capsules.
