The inhibition of antisense RNA retroviral vector of mTOR on proliferation of vascular smooth muscle cells
- VernacularTitle:反义mTOR逆转录病毒载体对血管平滑肌细胞增殖的抑制作用
- Author:
Xinhua HU
;
Qiang ZHANG
;
Jun YANG
;
Chengwei LIU
;
Zhishen ZHANG
- Publication Type:Journal Article
- Keywords:
mTOR;
Muscle,Smooth,Vascular/pathol;
Cell Division
- From:
Chinese Journal of General Surgery
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of antisense RNA retroviral vector of mTOR on the proliferation of vascular smooth muscle cells(VSMC).Methods The conservative region of mTOR gene was inserted into pLXIN reversedly,then the vector was packaged in PT67 cells by transfection with lipofectamine and(transfected) to VSMC.The efficiency of antisense inhibition was verified,and the changes of p70S6k and(4E-BP1) were also determined at the same time.The proliferation activity was determined by flowcytometry and MTT.Results After the vector was successfully transfected to the cells.pLXIN-A-mTOR vector could(efficiently) decrease mRNA and protein expression of mTOR and p70S6k of the cell,while the expression of 4E-BP1 increased significantly.The cell cycle and proliferation activity of VSMC was also stunned in phase G_1.Conclusions The mTOR antisense retroviral vector,pLXIN-A-mTOR,has been constructed(successfully),which can significantly inhibit the proliferation of VSMC.
