Construction of short hairpin RNA expression plasmid and its inhibition of survivin expression
- VernacularTitle:Survivin shRNA表达质粒的构建及其抑制survivin表达的初步研究
- Author:
Hanbin SHEN
;
Qichang ZHENG
;
Guobin WANG
;
Daoda CHEN
;
Xiaoming LU
- Publication Type:Journal Article
- Keywords:
Gallbladder Neoplasms/pathol;
Survivin Protein;
Tumor Suppressor Proteins
- From:
Chinese Journal of General Surgery
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibit effect of survivin short hairpin RNA on the survivin mRNA and protein expression of gallbladder carcinoma cells.Methods Human gallbladder carcinoma cells(GBC-SD) were transfected with recombinant plasmid.RT-PCR and Western blot were used to analyze the changes in(expression) levels of survivinmRNA and protein.Results The size of the PCR product was 350bp.DNA(sequencing) showed that the sequence of recombinant vector pshRNA-survivin was successfully constructed and suppressed the expression of(GBC-SD) survivinmRNA.Conclusions The recombinant plasmid constructed can inhibit the expression of survivinmRNA in transfected cells.This provides a new method and material for the biological therapy of cancer.
