Cloning, expression and identification of chlamydial GPIC capsid Vpl protein
- VernacularTitle:衣原体GPIC噬菌体衣壳蛋白Vp1的克隆、表达和鉴定
- Author:
Quanzhong LIU
;
Weifeng YAO
;
Manli QI
;
Yan LI
;
Huiping WANG
;
Jinqun TIAN
- Publication Type:Journal Article
- Keywords:
Chlamydiaphage;
Vp1 protein;
expression
- From:
Chinese Journal of Dermatology
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone, express, and identify chlamydial GPIC capsid Vpl gene and pro- tein. Methods The complete sequence of Vp1 gene from?CPG1 phage was amplified. The amplicor was cut by restriction endonuclease, linked to plasmid vector, and transformed into E. coli.The expressed pro- tein of recombinant Vp1 was purified and identified. Results The recombinant 1 661 bp gene was se- quenced and proved to be?CPG1 Vp1 by searching Genebank. A 62 kDa capsid protein was expressed and confirmed by SDS-PAGE and Western blot. Conclusion The recombinant Vp1 seems to be a highly con- served and specific marker for chlamydial phage.
