The study of in vitro culture conditions for induction of human neural stem cells to neuron like cells
- VernacularTitle:人胚神经干细胞向神经元样细胞诱导分化的研究
- Author:
Gang CHEN
;
Shangzhen QIN
;
Lianting MA
;
Ting LEI
;
Li PAN
;
Delin XUE
- Publication Type:Journal Article
- Keywords:
Stem cells;
Nervous system/cytology/ embryology;
Neurons;
Cell differentiation
- From:
Journal of Chinese Physician
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the in vitro culture conditions for neural stem cells to differentiate into neuron-like cells.Methods Neural stem cells were induced to differentiate by a medium containing bFGF and heparin through adhesive culture.Immunohistochemistry was used to identify the expressions of NSE and GFAP after differentiation.Results Positive cells for NSE were 47.3%?1.7% on the 7th day after induction.The number of NSE positive cells in the induction group was 3 times as many as that in the control group(P
