The effect of Tet-on gene regulated HIF-1?expression on hepatoma cell apoptosis in vitro
- VernacularTitle:Tet-on基因表达系统调控HIF-1?表达对肝癌细胞凋亡的影响
- Author:
Zongquan XU
;
Xiaoping CHEN
;
Wanguang ZHANG
;
Changhai LI
;
Gaopeng LI
- Publication Type:Journal Article
- Keywords:
Carcinoma, hepatocellular;
Apoptosis;
Hypoxia-inducible factor-1?;
Tet-on gene expression system
- From:
Chinese Journal of General Surgery
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Tet-on gene regulated HIF-1?expression on cell apoptosis of hepatoma cells in vitro. Methods The apoptosis of hepatocellular carcinoma cell lines HePG2 was measured after HIF-1?expression was activated in HePG2 in vitro by Tet-on expression system. Results The amplified products were confirmed as the cDNA of HIF-1?by DNA sequencing, and obtained pTHE-HIF-1?was verified by endonuclease digestion. The HePG2Tet-on cells express HIF-1?constantly. After being incubated under different concentrations of doxycycline for 48 h, HIF-1?gene could inhibit HePG2 cell apoptosis. The apoptosis rates were 59. 6%、50. 9%、38. 1%、30. 5%、23. 9%、18. 3% respectively when the concentrations of doxycycline were 0?g/ml,0. 02?g/ml,0. 2?g/ml、1?g/ml、2?g/ml,5?g/ml (P
