An in vitro study for the effect of newly constructed replication-selective adenovinis CNHK600-p53 on hepatocellular carcinoma cell lines PLC/PRF5
- VernacularTitle:增殖性腺病毒CNHK600-p53的构建及对肝癌细胞抑制的体外实验研究
- Author:
Jicheng DUAN
;
Qijun QIAN
;
Haiyan YUE
;
Li SHEN
;
Kai LIU
;
Jiahe YANG
;
Mengchao WU
- Publication Type:Journal Article
- Keywords:
Liver neoplasms;
Genes,p53;
Drug resistance;
Gene therapy
- From:
Chinese Journal of General Surgery
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a new replicating adenovirus vector CNHK600-p53 carrying anti-tumor gene p53 and investigate its effect on hepatocarcinoma cell line PLC/PRF5. Methods The methylthiazolyl tetrazolium assay (MTT) was used to observe the killing effect of Fluorouracil, Mitomycin and Epirubicin alone or in combination with adenovirus CNHK600-p53 on PLC/PRF5. Results When 5-Fu at concentration of 400?g/ml, the inhibition rate was (65?4. 2) % ; with MMC at 1?g/ml, the rate was (41?1.9)%; and it was (65?1.8)% when EPI at concentration of 10?g/ml. With PLC/PRF5 infected by adenovirus CNHK600-p53 ( MOI = 0. 625) , the inhibition rate was significantly increased to (89?5. 3)%,(60?2.3)% and (75?1.5)% respectively; When MOI was 0.3125, 0.625, 1.25, the inhibition rate in CNHK600-p53 group and Ad-p53 group was (27?2. 5)% , (30?3. 7)% , (61?4. 3)% and(4?2.7)%, (5?3.5)%, (16?4.5)% respectively. Conclusion For hepatocarcinoma cell line PLC/PRF5 the effect of CNHK600-p53 is stronger than Ad-p53.
