Effect of ischemic preconditioning on expression of nitric oxide synthase in rat small-for-size liver graft
- VernacularTitle:缺血预处理对大鼠小体积供肝一氧化氮合酶的影响及意义
- Author:
Genshu WANG
;
Guihua CHEN
;
Minqiang LU
;
Bin HU
;
Wenzhang SI
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Ischemic preconditioning;
Nitric-oxide synthase
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of ischemic preconditioning(IPC) on expression of nitric oxide synthase(NOS) in rat smallfor-size liver graft and its significance.METHODS: Sixty SD rats were randomly divided into 3 groups(n=10 pairs/group): nonwarm ischemia group(NWI);warm ischemic group(WI);and ischemic preconditioning group(IPC).The models of rat small-for-size liver transplantation were set up by two-cuff technique.Expression of eNOS mRNA and iNOS mRNA in hepatic tissue were detected by fluorescence-quantitating-PCR.RESULTS: Heptic expression of eNOS mRNA post-IPC was higher than that pre-IPC(P0.05).It was higher in IPC group than that in NWI and WI group(P
