Effects of chronic hypoxia on intracellular calcium concentration in pulmonary arterial smooth muscle cells
- VernacularTitle:慢性缺氧对大鼠肺动脉平滑肌细胞胞内钙的影响及其机制研究
- Author:
Huimin WU
;
Yongjian XU
;
Zhenxiang ZHANG
;
Shuyun XU
- Publication Type:Journal Article
- Keywords:
Anoxia;
Pulmonary artery smooth muscle cells;
Calcium;
Calcium channels,L-type
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of chronic hypoxia(CH) on the intracellular calcium([Ca~(2+)]i) in pulmonary artery smooth muscle cells(PASMCs) and the role of L-type calcium channel and calcium store. METHODS: The rat chronic hypoxia model was set up and intervene the PASMCs with normal PSS,calcium-free PSS,nifedipine,and heparine respectively.The resting [Ca~(2+)]i was determined with the Fura-2/AM calcium imaging technique.RESULTS:(1) The [Ca~(2+)]i in CH group in normal PSS was higher than that in control group in normal PSS(P
