Expression of B lymphocyte stimulator in peripheral blood mononuclear cells in individuals with SLE and effect of dexamethasone on its expression
- VernacularTitle:SLE患者PBMC表达B细胞刺激因子及地塞米松对其表达的影响
- Author:
Yujin YE
;
Hanshi XU
;
Duorong XU
;
Liuqin LIANG
;
Xiuyan YANG
;
Zhongping ZHAN
;
Fan LIAN
;
Peida YIN
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
B-lymphocytes;
Dexamethasone
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine the expression of membrane-bound B lymphocyte stimulator((BLyS)) and its mRNA in peripheral blood mononuclear cells(PBMCs) from individuals with systemic lupus erythematosus(SLE),and to investigate the effect of dexamethasone on(BLyS) expression.METHODS: PBMCs were obtained from 25 individuals with SLE(mean age of 31.40?14.23) and 20 female healthy volunteers(mean age of 28.20?10.36).They were randomized into dexamethasone((1 ?mol/L)) group and media group.PBMCs were gathered at 0,6,12 and 24 h for(BLyS) mRNA assessment using reverse transcription-PCR(RT-PCR).PBMCs were also collected at 72 h for membrane-bound(BLyS) protein detection using flow cytometry(FACS) and direct immunofluorescence.RESULTS:(1) The expression of(BLyS) mRNA and membrane-bound protein were significantly higher in PBMCs from individuals with SLE than that in PBMCs from healthy controls(0.40?0.18 vs 0.27?0.20,P
