Expression of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in hepatocellular cell lines transfected by PRL-2 gene
- VernacularTitle:PRL-2基因转染对肝细胞基质金属蛋白酶及其抑制剂表达的影响
- Author:
Chao CHENG
;
Ailin GUO
;
Weikang WU
;
Honghe LUO
;
Fotian ZHONG
;
Meng ZHANG
- Publication Type:Journal Article
- Keywords:
Hepatocytes;
Protein-tyrosine-phosphatase;
Gene transfection;
Matrix metalloproteinases
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the possible mechanism of PRL-2 in invasive metastasis of tumors.METHODS: The PRL-2 vector was transfected into CL1 cell with lipofectamine reagent,the transfectants were selected by growth in the medium supplemented with G418.Zymographic analysis of metalloproteinases(MMPs) activity was performed,RT-PCR was used to determine the mRNA levels of(MMP-2),MMP-9,TIMP-1 and TIMP-2,the protein levels of(MMP-2),MMP-9,TIMP-1 and TIMP-2 were analyzed by Western blotting.The effects of the special inhibitor of PRL-2 on transfected cells were also observed.RESULTS: The stable cell line selected by G418 was identified by RT-PCR and Western blotting.More abundance of MMP-2,MMP-9 and its activated type were secreted by the CL-1-PRL-2 cells than untransfected cells and transfected vector cells(P
