Effect of curcumin on the proliferation and apoptosis in LNCap cells
- VernacularTitle:姜黄素对前列腺癌细胞LNCaP增殖的影响
- Author:
Lei YANG
;
Lianying ZHANG
;
Weiwen CHEN
;
Xiaoyan HU
;
Jianye ZHANG
;
Fuai CUI
- Publication Type:Journal Article
- Keywords:
Prostatic neoplasms;
Curcumin;
Apoptosis;
LNCacp cells
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effect of curcumin on the proliferation and apoptosis of prostate cancer cell line LNCap.METHODS: LNCap cells were treated with different doses(10 ?mol/L,20 ?mol/L,30 ?mol/L,40 ?mol/L) of curcumin and its effects were analyzed in cell growth and apoptosis by microscope,MTT colorimetric assay and flow cytometry.The expression of prostate specific antigen(PSA) was measured by AXSYM~(TM) system-chemical luciferase methods and expression of androgen receptor (AR) was detected by Western blotting.RESULTS: The results showed that curcumin inhibited the proliferation of LNCap cells.The cell growth was inhibited by curcumin in a dose dependent manner and the optimal dose and time was 40 ?mol/L,24 h.Curcumin induced apoptosis in LNCap cells,the most dramatic dose was 40(?mol/L) curcumin,at this dose the apoptosis rate was 9.23%. Curcumin inhibited the expression of PSA in LNCap cells and the most dramatic dose and time was 40 ?mol/L,24 h.The PSA in this group was 20% of the control group.Curcumin inhibited the expression of AR on prostate cancer cells.CONCLUSION: Curcumin decreases proliferation and induces apoptosis in LNCap cells in a dose-dependent manner.Curcumin also inhibites the expression of PSA and AR on LNCap cells.
