Effects of VPA in combination with HA14-1 on a Bcl-2 overexpressed leukemia cell line BALL-1
- VernacularTitle:VPA联合HA14-1对Bcl-2高表达的BALL-1细胞作用的实验研究
- Author:
Hongman XUE
;
Chun CHEN
;
Jianyu ZHANG
;
Jinhua ZHANG
;
Wenyi LI
- Publication Type:Journal Article
- Keywords:
Valproic acid;
HA14-1;
Bcl-2;
Leukemia;
NOD/SCID mice
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effects of valproic acid(VPA) and HA14-1 on Bcl-2 overexpressed leukemia cells in vitro and in vivo.METHODS:(1) Cells were divided into control group,HA14-1 group,VPA group and HA14-1+VPA group.The apoptotic rate,the mean fluorescent index(MFI) of Bcl-2,the levels of caspase 3,8 and 9 were detected with FCM.(2) 24 h after transplantation with BALL-1,the NOD/SCID mice were divided into 4 groups as(1),then the survival time was compared.The expression of CD19 in the peripheral blood cells,bone marrows,livers,spleens and lungs of each group was detected.RESULTS:(1) The apoptotic rate in HA14-1+VPA group was(76.5?6.9)%,this was significantly elevated compared to the data in other groups(P
