Adenovirus mediated MDA-7/IL-24 gene transfer selectively kills hepatocellular carcinoma lines HepG2
- VernacularTitle:腺病毒介导MDA-7/IL-24选择性杀伤肝癌细胞HepG2的研究
- Author:
Congjun WANG
;
Xinbo XU
;
Jilin YI
;
Kun CHEN
;
Jianwei ZHEN
;
Jianping ZENG
;
Ronghua XU
;
Weiyu WANG
;
Zaide WU
- Publication Type:Journal Article
- Keywords:
Liver Neoplasms,Experimental/ther;
Gene therapy;
Hepatocellalar Carcinoma HepG2;
Gene,MDA-7/IL-24
- From:
Chinese Journal of General Surgery
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the selective killing effect of MDA/IL-24 on human hepatocellular carcinoma line HepG2 in vitro and provide a theoretical basis for gene therapy of hepatocellular carcinoma.Methods The MDA-7/IL-24 gene was transfected into human hepatocullular carcinoma cell line HepG2 and normal liver cell line L02 with a replication-incompetent adenovirus vector.The mRNA and protein expression of MDA7/IL-24 in HepG2 and L02 cells was examined by RT-PCR and ELISA assay respectively.MTT assay and flow cytometry were used to study tumor cell proliferation and cell cycle in vitro.Hoechst and Annexin-V and PI staining were studied to indicate the apoptosis.Results RT-PCR confirmed that the exogenous MDA-7/IL-24 gene was expressed in HepG2 and L02 cells.The protein product was confirmed by assay of the supernatant with ELISA.MTT and apoptosis test indicated MDA-7/IL-24 induced growth suppression and cell apoptosis of the HepG2 cell in vitro but not in cell line L02,and cell cycle test revealed MDA-7/IL-24 could block HepG2 cell in G2/M but not in L02.Conclusions MDA-7/IL-24 selectively induces growth suppression and apoptosis in lines HepG2 in vitro but not in L02 cell,which indicates that adenovirus mediated MDA-7/IL-24 can be an excellent tool for gene therapy in hepatocellular carcinoma.
