Effects of paeonol on the tyrosinase activity and melanogenesis in co-culture model of human melanocytes and keratinocytes
- VernacularTitle:丹皮酚在体外对人黑素细胞酪氨酸酶活性及黑素生成的影响
- Author:
Shihai XIE
;
Zhiqiang CHEN
;
Jin BU
;
Jianming GAO
;
Wuqing ZHOU
;
Lingjun LI
;
Pengcheng MA
- Publication Type:Journal Article
- Keywords:
Paeonol;
Melanocyte;
Keratinocytes
- From:
Chinese Journal of Dermatology
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of paeonol on the tyrosinase activity and melanogenesis in co-culture model of human melanocytes and keratinocytes. Methods Melanocytes and the co-culture model of human melanocytes and keratinocytes were cultivated and the proliferation of melanocytes and the co-cultures was measured by MTT colorimetric assay. The tyrosinase activity and melanin level were measured by enzymic method. Results The melanin synthesis and tyrosinase activity were markedly suppressed by paeonol in a dose-dependent manner at the concentrations of 50?mol/L, 100?mol/L, and 200?mol/L in both melanocytes and co-cultures. The significant stronger suppression was observed with 100?mol/L and 200?mol/L of paeonol than that with controls (P
