Inducement of Dendritic Cells Derived from Cord Blood Mononuclear Cells and CTLs Antitumor Immunity in Vitro
- VernacularTitle:脐血单个核细胞体外诱导树突状细胞联合CTLs抗肿瘤效应的研究
- Author:
Xicai WANG
;
Zhiping WU
;
Yongxin JIANG
;
Xiaosan SHU
;
Congguo JING
;
Yongchuen ZHOU
- Publication Type:Journal Article
- Keywords:
Cord blood mononuclear cells;
Dendritic cells;
Cytotoxic T lymphocytes;
Antigen presenting cells
- From:
Journal of Kunming Medical University
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the way of inducing dendritic cells from precursor in human cord blood and its role in antitumor immunity.Methods Cord blood was collected under sterile condition and the cord blood mononuclear cells were separated by centrifugal in density gradient.CBMCs were cultured with GM-CSF+IL-4+TNF-?and cell phenotype was analyzed with CD1a、CD83 antibody by using indirect immunofluorescence assays.The effects of DCs pulsed with tumoral antigens on cytotoxic T lymphocytes(CTLs) inducement and growth inhibition of YTMLC cells were assayed.Results Our results indicated that DCs precursors in human cord blood can be induced to differentiate in the medium containing GM-CSF、IL-4 and TNF-?.The cells with typical morphological properties of DCs were observed at the 7th day.At that time,(20.8?1.62)%CD1a+ cells were obtained.After incubation with tumor cytolysis antigen,the DCs can activate the CTLs to become tumor specialized CTLs,which had shown significantly inhibition on growth of YTMLC tumor cell line.Conclusion The precursors in human cord blood can be induced to functional DCs which activate T lymphocyte to become tumor specialized CTLs.
