Inhibitory effects of siRNA targeting protein kinase CK2?on the invasion of laryngeal carcinoma cells
- VernacularTitle:靶向蛋白激酶CK2?的siRNA抑制喉癌细胞侵袭的实验研究
- Author:
Jianting WANG
;
Shusheng GONG
;
Yingpeng LIU
;
Guangli CHEN
- Publication Type:Journal Article
- Keywords:
Laryngeal Neoplasms;
Carcinoma, Squamous Sell;
Protein Kinase;
RNA Interference
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To construct siRNA eukaryotic expression vector targeting protein kinase CK2?and to investigate its inhibitory effect on invasion of the HEp-2 cell line in human laryngeal carcinoma. METHODS siRNA expression vector psiRNA-hH1neo-CK2 targeting protein kinase CK2?was constructed by gene recombination,and then was transfected into the HEp-2 cells by lipofectamine methods. Protein kinase CK2?mRNA and protein of the transfected cells were detected by reverse transcription polymerase chain reaction(RT-PCR) and Western blot,respectively. The invasion of the transfected cells was measured by Boyden chamber.SP method was used to examine the expressions of MMP2 and TIMP2 protein of the transfected HEp-2 cells. RESULTS Protein kinase CK2?siRNA expression vector was successfully constructed by gene recombination. Compared with non-specific interfering groups and blank groups, protein kinase CK2?mRNA and protein were significantly decreased respectively in the psiRNA-hHIneo-CK2 groups(P
