The inhibitory expression of VEGF in antisense blocking of PTTG gene on human cholangiocarcinoma cell line QBC939
- VernacularTitle:反义抑制PTTG对胆管癌细胞株QBC939中VEGF表达的影响
- Author:
Ling CHEN
;
Huihuan TANG
;
Chao FEN
;
Yanguang WEN
;
Zhiyuan JIAN
- Publication Type:Journal Article
- Keywords:
VEGF;
PYYC;
Tumor Cells,Culturred;
Bile Duct Neoplasms/gene
- From:
Chinese Journal of General Surgery
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of expression of pituitary tumor transforming gene(PTTG) on the expression of inhibitory of vascular endothelial growth factor(VEGF).Methods The constructed(recomibinant) vector,pcDNA3.1-PTTGas that contained full-length antisense PTTG,was transfected into the cholangiocarcinoma cell line QBC939 in different quantity.After 48h incubation,the variation of expressions of PTTG and VEGF mRNAs and proteins were observed by RT-PCP and Western-blot method.Results(After) successful transfecting the recomibinant vector into QBC939,the mRNAs and proteins of PTTG and VEGF were all inhibited.Linear correlation analysis showed positive correlation between descendent extents of PTTG and VEGF(P
