Overexpression of Smad7 inhibits TGF-?-induced Smad2 mRNA and protein expression in peritoneal mesothelial cells
- VernacularTitle:上调表达Smad7显著抑制TGF-?介导的大鼠腹膜间皮细胞Smad2的表达
- Author:
Wenjuan DUAN
;
Xueqing YU
;
Xianrui DOU
;
Qiongqiong YANG
;
Xiaoyan LI
- Publication Type:Journal Article
- Keywords:
Peritoneal mesothelial cells;
Transforming growth factor beta;
Protein Smad2;
Protein Smad7
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of Smad7 in the Smad2 expression induced by transforming growth factor-?_1(TGF-?_1) in rat peritoneal mesothelial cells(PMCs).METHODS: Rat PMCs were cultured at different doses of TGF-?_1 (0,1.25,2.5,10 ?g/L) for different time(0,5,15,30,60,120 min).PCDNA3-Smad7 was then transfected into cultured rat PMCs by lipofectamine,and the cells were stimulated like the above.Endogenous Smad2 and Smad7 expression was evaluated by RT-PCR and Western blotting.RESULTS: TGF-?_1 induced increase in Smad2 mRNA and protein expression at 5 min,peaked at 30 min,and declined to baseline levels at 120 min, which was in a time-dependent manner.TGF-?_1 also induced Smad7 mRNA expression at 5 min,and then declined,down to the lowest at 30 min,but at 60 min it increased again.Smad2,Smad7 mRNA and protein expression induced by TGF-?_1 were also dose-dependent.After transfection,overexpressions of Smad7 mRNA and protein in rat PMCs were observed,which did not decline with time.The expression of Smad2 mRNA significantly decreased by 33%,56%,67%,71%,63% and 57%(P
