Expression of TGF-?_1 and Smad2/3 in kidney of STZ-induced diabetic nephropathy rats
- VernacularTitle:转化生长因子胞内信号蛋白Smad2/3在糖尿病大鼠肾脏表达的动态观察及意义研究
- Author:
Qin YANG
;
Rujia XIE
;
Bing HAN
;
Ying XIAO
;
Ting YANG
;
Li FANG
;
Yiguo LONG
;
Guozhong ZHANG
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathies;
Transforming growth factor bate;
Protein Smad2/3;
Signal transduction;
Rats
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the role of TGF-?/Smad pathway in the development of renal fibrosis in diabetic nephropathy.METHODS: Rats were induced to diabetic nephropathy by using tail intravenous injection of STZ.The expression of TGF-?_1,Smad2/3 protein and mRNA in kidney were examined at 2,4,8 and 16 weeks after STZ induction.CTGF,collagen-Ⅲ,PAI-1 mRNA expression in kidney at 16 weeks of STZ-induced diabetic nephropathy and normal rats were studied by RT-PCR.RESULTS: Weak TGF-?_1,Smad2/3 protein were detected in normal renal tissues while strong TGF-?_1,Smad2/3 staining were observed in renal tissues of diabetic nephropathy(0.057?0.030/0.223?0.040;0.017?0.010/0.153?0.010,respectively,P
