Inhibitory effects of CCK-8 on NF-?B activities stimulated by LPS in rat PIMs
- VernacularTitle:CCK-8抑制LPS作用下大鼠肺间质巨噬细胞NF-?B活性的cAMP-PKA通路研究
- Author:
Weijuan GAO
;
Shunjiang XU
;
Bin CONG
;
Shujin LI
;
Chunling MA
;
Jinrong XU
;
Yuxia YAO
;
Zhenyong GU
- Publication Type:Journal Article
- Keywords:
Cyclic AMP-dependent protein kinases;
Cholecystokinin;
Macrophages,pulmonary interstitial;
NF-kappa B;
Lipopolysaccharides
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the inhibitory effects of cholecystokinin octapeptide(CCK-8) on nuclear factor-?B(NF-?B) activities stimulated by lipopolysaccharide(LPS) by using forskolin,the activator of adenylate cyclase,and PKA inhibitor H-89 in rat pulmonary interstitial macrophages(PIMs).METHODS: PIMs were isolated and purified.EMDA was applied to detect NF-?B activities and Western blotting was used to analyze the I?B-? protein level in rat PIMs.RESULTS: The NF-?B activity was not detected in normal control rat PIMs.The NF-?B activity in LPS-treated rat PIMs was obviously higher than that in control group(P0.05).The NF-?B activity in CCK+LPS group and LPS+Fsk group were obviously lower than that in LPS group(P
