Integrin ?_3-FAK pathway is involved in neointima formation
- VernacularTitle:整合素?_3-粘着斑激酶信号途径的活化参与大鼠血管新生内膜的形成
- Author:
Jinkun WEN
;
Mei HAN
;
Yunhui CHENG
;
Changchun YANG
;
Zhimin LIU
- Publication Type:Journal Article
- Keywords:
Tunica intima;
Extracellular matrix;
Integrins;
Focal adhesion kinase
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate whether the expression of integrin ?_3 and the activation of focal adhesion kinase(FAK) are involved in neointima formation after de-endothelium.METHODS: The model of intima hyperplasia was prepared by balloon injury.The levels of osteopontin(OPN),integrin ?_3 and FAK in vascular tissue were detected by Western blotting and immunohistochemistry.RESULTS: There were similar expression patterns in OPN,integrin ?_3 and FAK following balloon injury.The levels of three proteins were markedly increased 3 days after operation and reached the peak at 7th day.The increased FAK was mainly the phosphorylated form.The migration and proliferation of vascular smooth muscle cells was associated with the increase in the expression of integrin ?_3 and FAK,and was parallel with rapid turnover of extracellular matrix(ECM).CONCLUSION: The interaction of cells with ECM mediated by OPN and integrin ?_3 is essential for migration.The integrin ?_3-FAK pathway is involved in neointima formation.
