Foam cells can be induced by oxidized low density lipoprotein in human monocyte-derived dendritic cells
- VernacularTitle:氧化修饰低密度脂蛋白可诱导人单核细胞源树突状细胞形成泡沫细胞
- Author:
Chun LIANG
;
Yukun LUO
;
Dong HUANG
;
Qingzhe JIA
;
Congfeng XU
;
Keqiang WANG
;
Zonggui WU
;
Junbo GE
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Arteriosclerosis;
Lipoproteins,LDL;
Foam cells
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The purpose of this study is to investigate the mechanisms related to oxidized low-density lipoprotein(ox-LDL) and dendritic cells(DCs) in the process of atherosclerosis.METHODS: Human DCs were prepared from human CD14~+ peripheral blood monocytes using rhGM-CSF((100 ?g/L)) and rhIL-4(40 ?g/L).Cells were incubated with(100 mg/L) native or oxidized LDL for 72 h.The formation of foam cells was investigated by electron microscopy and oil red O staining.Phenotypic and immune functional assays were used with FACS,FITC-dextran phagocytosis,allogeneic mixed T lymphocytes reaction and secretion of Th1/Th2(IL-12/IL-2) cytokines were also conduced.RESULTS: DCs treated with ox-LDL,but not native LDL were induced into foam cells after cultured for 72 h.Compared with native LDL,ox-LDL-treated DCs were less potent in FITC-dextran phagocytosis.ox-LDL promoted allogeneic T cells proliferation.Moreover,ox-LDL upregulated CD80(72.4? 9.6 vs 89.5?10.1,P
