Changes of expression of scavenger receptor class B typeⅠ(SRBI) in phorbol 12-myristate 13-acetate differentiated U937 cells
- VernacularTitle:U937细胞向巨噬细胞分化过程中清道夫受体BI(SR-BI)表达的变化
- Author:
Yang PENG
;
Jin ZHANG
;
Li LI
;
Xin MENG
;
Yijun ZHOU
;
Yu GAN
;
Difei WANG
;
Shuai HOU
- Publication Type:Journal Article
- Keywords:
U937 cells;
Macrophages;
Receptors,scavenger;
Arteriosclerosis;
Foam cells
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the changes of expression of SR-BI in phorbol 12-myristate 13-acetate(PMA) differentiated U937 cells.METHODS: U937 cells were cultured with 100 nmol/L PMA in order to differentiate the cells to macrophages.Immunocytochemical method,Western blotting analysis and reverse transcription polymerase chain reaction(RT-PCR) were used to detect SR-BI protein and mRNA during differentiation.RESULTS: Immunocytochemistry showed that after exposure of U937 cells to PMA for 24,48,72 hours,the values of SR-BI protein expression in U937 cells were 15.94?3.56,27.86?4.39 and 9.08?2.37,with the first two higher than that in undifferentiated cells(7.76?1.74,P0.05) increment in the expression of SR-BI protein compared with U937 monocytes.RT-PCR showed that relative SR-BI mRNA expression in different group was 0.112?0.006,0.235?0.014,0.344?0.140 and 0.138?0.010,respectively.CONCLUSION: SR-BI protein and mRNA were increased after differentiation,reached a peak at 48 hours,and decreased at 72 hours.High expression levels of SR-BI in U937 macrophages following PMA differentiation may be correlated with foam cell formation.
