Preliminary study of endothelial progenitor cells formed vessel-like structure in vitro
- VernacularTitle:内皮祖细胞在体外培养成血管样结构的初步观察
- Author:
Deye YANG
;
Huaiqin ZHANG
;
Kangting JI
;
Xiaoyan HUANG
;
Lixin XU
;
Houyan SONG
- Publication Type:Journal Article
- Keywords:
Endothelial progenitor cells;
Cell culture;
Tissue engineering
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: Endothelial progenitor cells(EPCs) are a group of stem cells/progenitor cells,which exist in postnatal body and can be of specially homing to the foci of angiogenesis and then differentiate into endothelial cells.This investigation was to study the method for culturing endothelia progenitor cells(EPCs) in vitro,and to observe its feasibility and condition formed vessel-like structure.METHODS: The cells were isolated from born marrow,peripheral blood,umbilical cord blood or spleen in different laboratories.The EPCs derived from human umbilical cord blood and rabbit peripheral blood were cultured in vitro through adhesion selection and were differentiated into endothelial cells under the induction of special cytokines.The expression of CD34,VEGFR-2,AC133 and VE-cadherin were detected by fluorescence-activated cell sorting.The endothelial cell lineage was confirmed by DiI-ac-LDL up-taking and Ⅷ factor immunocytochemistry.RESULTS: The EPCs derived from human umbilical cord blood and rabbit peripheral blood were cultured in vitro successfully,forming cord-like and tube-like structure.The EPCs derived from rabbit peripheral blood differentiated more mature and formed vessel-like structure.CONCLUSION: The EPCs derived from human umbilical cord blood and rabbit peripheral blood formed vessel-like structure in vitro.EPCs may be a potential resource of vessel tissue engineering.
