Effects of dendritic cells co-cultured with CIK cells on renal carcinoma cells
- VernacularTitle:抗原致敏DC与CIK共培养体外抗肾癌效应的研究
- Author:
Hailun ZHAN
;
Xin GAO
;
Jianguang QIU
;
Yubin CAI
;
Jie SITU
;
Xingqiao WEN
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Cytokines;
Killer cells;
Kidney neoplasms
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of CIK cocultured with DC that pulsed with RCC antigen on renal carcinoma cells.METHODS: DC and CIK cells were generated respectively by cytokines from PBMC of healthy blood donor.Cell surface markers were analyzed by flow cytometry.Then CIK were cocultured with autologous DC that was(or not) pulsed with RCC antigen(786-0 cells).Cytotoxic activity against 786-0 or PC3 cells was measured by MTT assay under three different conditions: CIK cocultured with DC which was pulsed with 786-0 antigen(group A);CIK cocultured with DC which is not pulsed with 786-0 antigen(group B);CIK without DC(group C).RESULTS: The cytotoxic activity of three groups against 786-0 cells was(70.64?8.26)%,(53.40?7.33)%,(46.64?6.01)%,respectively(E/T=(20∶1)).Significant differences between group A and group B or between group A and group C were observed(P
