Differentiation of fetal mesenchymal stem cells into pancreatic islet-like clusters in vitro
- VernacularTitle:胎儿骨髓间充质干细胞体外定向诱导分化为胰岛样细胞
- Author:
Xiufeng HUA
;
Wei WANG
;
Haiyan WANG
;
Peiwen LIAN
;
Shouxin ZHANG
;
Shulin CHEN
;
Wei GAO
;
Jianyuan LI
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cell;
Bone marrow cells;
Islets of langerhans
- From:
Journal of Chinese Physician
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study and to optimize culture conditions of islet-like cells induced in vitro from fetus bone marrow(BM) mesenchymal stem cells(MSCs).Methods BM was obtained from miscarried human fetus.The MSCs between three to eight passages were used to differentiate into islet-like clusters-through three stages of culture supplemented with 2mercaptoethanol,epidermal growth factor(EGF),basic fibroblast growth factor(bFGF),B_(27) and nicotinamide.Results The 2nd stage cells expressed nestin and/or panceatic and duod-enal homeobox 1(PDX-1),and the 3rd stage cells formed islet-like clusters expressing insulin and glucagon together with positive dithizone staining.Specific insulin secretion could be detected(81.3?23.6?u /ml) from differentiated MSCs which have the capacity to respond to different glucose concentrations.Conclusion Fetal bone marrow MSCs can be differentiated into pancreatic islet-like clusters,and 20mmol/L nicotinamide could be the optimal concentration in culture.
