The effect and mechanism of caveolin-1 upregulation on the proliferation of MGC803 cell line
- VernacularTitle:Caveolin-1对MGC803细胞增殖的影响及机制
- Author:
Hongmei LUO
;
Shengsong TANG
;
Liming TAN
;
Duanfang LIAO
;
Pengke YAN
;
Yueshun LIU
;
Zhifeng LONG
- Publication Type:Journal Article
- Keywords:
Caveolins;
Stomach neoplasms;
Cell proliferation
- From:
Journal of Chinese Physician
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of caveolin-1 gene expression on the proliferation of human gastric adenocarcinoma cells,and to explore the possibility for its future usage in gene therapy.Methods The full-length caveolin-1 gene was stably transfected into the MGC803 cell line by lipofectin.The Pcl neo vector was transfected at the same time as mock control.The expression of caveolin-1 was detected by Western blot in both the caveolin-1 gene transfected MGC803 cells and the controls.The cell cycle was analyzed by flow cytometry.Results After transfected with caveolin-1,MGC803 cells significantly up-regulated the expression of caveolin 1 and extended their doubling time.The cell proliferation was inhibited and the cell cycle was arrested in the G_0/G_1 phase.Conclusion Caveolin-1 can inhibit the proliferation of MGC803 cells and induce cell cycle arrest in G_0/G_1 phase.
