Effect of Astilbin on expressions of perforin and granzyme B in activated T cells of mouse heart transplantation model with acute rejection
- VernacularTitle:落新妇甙对心脏移植排斥反应中活化T细胞穿孔素和颗粒酶B表达的影响
- Author:
Sihai GAO
;
Tao CHEN
;
Tiechen PANG
;
Ping LI
;
Qinzi XU
;
Ligang LIU
;
Dingwei SONG
;
Jinpin ZHAO
- Publication Type:Journal Article
- Keywords:
Ultrasonography,doppler,color;
Heart transplantation;
Graft transplantation;
T-lymphocytes;
Membrane glycoproteins;
Serine endopeptidases
- From:
Journal of Chinese Physician
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of astilbin on expressions of perforin and granzyme B in activated T cells of mouse heart transplantation model with acute rejection.Methods Cardiomyocytes of BALB/C mouse and spleen cells of C57BL/6 mouse were harvested and made into single cell suspensions.The cardiomyocytes(2?10~5 ml~(-1))as stimulators and spleen cells(1?10~6 ml~(-1)) as responsers were mixed and cultured.The model of mouse heart transplantation with acute rejection in vitro was therefore established.There were two groups in the experiment.Control group is the mixed culture of the cardiomyocytes and spleen cells;Astilbin group is the mixed culture of the cardiomyocytes and spleen cells with astilbin(15?g/ml).Apoptosis of T cells were analyzed by TUNEL assay.The expressions of perforin and granzyme B were measured by RT-PCR.Results Apoptosis of activated T cells in Astilbin group was significantly increased than that of the control group(P
