Simultaneous Determination of Three Components of Saridon Tablet in Human Plasma by RP-HPLC
- VernacularTitle:反相高效液相色谱法同时测定散利痛片3组分的血药浓度
- Author:
Zan XIE
;
Ye JIANG
;
Yanrong LI
- Publication Type:Journal Article
- Keywords:
Saridon;
Caffine;
Acetaminophen;
Isopropylphenazone;
HPLC;
Blood drug concentration
- From:
China Pharmacy
2005;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method for simultaneous determination of caffine,acetaminophen and isopropylphenazone in human plasma after oral dose of saridon.METHODS:Hypersil Hypurity TM Advance was used as chromatographic column and the mobile phase was a mixture of0.01mol/L phosphoric acid-methanol-tetrahydrofuran(86∶12∶2).The detection wavelength was273nm.RESULTS:The calibration curves were linear in the ranges of0.39～100.0?g/ml for caffine,1.96～500.0?g/ml for acetaminophen,and1.17～300.0?g/ml for isopropylphenazone,respectively.The accuracy of all was higher than95.0%.The inter-day and intra-day RSD was less than10.0%.CONCLUSION:The present method is sensitive,accurate,convenient,and highly specific,which can meet the requirement in plasma determination of three components in Saridon tablet.
