Determination of Aristolochic Acid Ⅰ and Aristolochic Acid Ⅱ in Human Serum by HPLC
- VernacularTitle:高效液相色谱法测定人血清中马兜铃酸Ⅰ、Ⅱ的含量
- Author:
Guanghui ZHU
;
Zengshou WANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Aristolochic acid Ⅰ;
Aristolochic acid Ⅱ;
Serum concentration
- From:
China Pharmacy
2005;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for the determination of aristolochic acidⅠand aristolochic acid Ⅱin human serum.METHODS:The separation was performed on C 18 column(250mm?4.6mm,5?m).The mobile phase consisted of methanol-water-acetic(72∶27∶1)at a flow rate of1.0ml/min.The detection wavelength was250nm.RESULTS:The linear ranges for aristolochic acidⅠand aristolochic acid Ⅱ were0.84～13.50?g/ml(r=0.9997,n=5)and2.03～32.50?g/ml(r=0.9994,n=5),respectively.The lowest determination concentration of aristolochic acid Ⅰ and aristolochic acid Ⅱ were0.3?g/ml and0.1?g/ml,respectively.CONCLUSION:The method is simple and practicable,and it can provide basis for clinical diagnosis and treatment of aristolochic acid nephropathy.
