Molecular mechanisms involved in regulation of proliferation and apoptosis by Stat3 dominantnegative gene in colon cancer cells
- VernacularTitle:Stat3显性负性基因调控人结肠癌细胞增殖与凋亡的分子机制
- Author:
Yong ZHAO
;
Shan WANG
;
Yingjiang YE
;
Jing ZHOU
;
Kewei JIANG
;
Zhirong CUI
- Publication Type:Journal Article
- Keywords:
Colonic neoplasms;
Cell proliferation;
Apoptosis;
Transcription factor, STAT3;
Dominant-negative
- From:
Chinese Journal of General Surgery
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of transferring a dominant-negative Stat3 gene, Stat3?on colon cancer cells' proliferation and apoptosis in vitro. Methods Cell culture of human colon cancer cell line SW480 and transient transfection were used to evaluate the effect of transferring Stat3?to cancer cells. Cell proliferation, cell cycle and apoptosis were quantified by MTT and flow cytometry, respectively. The mRNA expression of Stat3's target gene cyclin D1 and bcl-xL was detected by reverse transcription polymerase chain reaction. Independent t tests were used for data statistics. Results 36 h after Stat3?plasmids transfection, proliferation of SW480 cells was significantly inhibited (t =5. 216,P = 0.006); cell proportion of G0/G1 phase increased from 40.37% to 67.25% and early apoptosis cells increased from 5. 34% to 24. 42% ; mRNA expression of cyclin Dl and bcl-xL declined significantly (t = 5.288,P=0.010;t=3.517,P=0.025). Conclusion Blocking Stat3 signaling pathway by transfection of Stat3?plasmid inhibits the proliferation and promotes apoptosis of colon cancer cells, which provides a experimental foundation of Stat3 targeted colon cancer gene therapy.
