Interference effect of nerve growth factor on apoptosis of retinal cells in experimental retinal detachment
- VernacularTitle:实验性视网膜脱离时神经生长因子对视网膜细胞凋亡的干预作用
- Author:
Xiaodong SUN
;
Xi ZHANG
;
Xun XU
- Publication Type:Journal Article
- Keywords:
Retinal detachment;
Nerve growth factor;
Cell death;
Disease models, animal
- From:
Chinese Journal of Ocular Fundus Diseases
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the interference effect of nerve growth factor (NGF) on apoptosis of retinal cells in experimental retinal detachment (RD). Methods Twenty seven Sprague-Dawely rats were selected, and the left and right eyes were in the experimental control group and NGF group, respectively. After the RD model was set up by subretinal injection with sodium hyaluronate, 5 ?l NGF (1 ?g?l) was injected into the vitreous body of the right eyes which were in the NGF group; 5 ?l PBS was injected into vitreous body of left eyes which were in the experimental control group. The injection was performed once every 4 days till the end of the observation period. The eye balls of the 27 rats were extrafted 1.5, 3, 6, 12 hours, 1 day, 2, 4, 8, 16, and 32 days after the RD model was established. Another 2 rats were selected as the normal control, which underwent none of the injections but eyeball extraction at the end of the observation period. TUNEL and transmission electron microscopy were used to detect the apoptosis of the retinal cells. Cell counts and statistical analysis were used to assess results. Results Typical apoptosis cells were observed in the early time of RD. Apoptosis was found in each retinal layers, especially in inner and outer nuclear layers. The number of apoptosis cells increased as the time of RD was prolonged(P
