Quantitative analysis of fetal DNA in maternal plasma
- VernacularTitle:定量检测孕妇血浆中胎儿DNA在产前诊断的应用
- Author:
Jing WU
;
Yanyi HUANG
;
Xiaoman CHEN
- Publication Type:Journal Article
- Keywords:
Pregnancy;
DNA;
Polymerase chain reaction;
Prenatal diagnosis
- From:
Chinese Journal of Perinatal Medicine
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop real-time quantitative PCR method for measuring the amount of fetal DNA in maternal plasma and the changing patterns of fetal and maternal DNA with the pregnant progress. Methods Fifty-eight women were recruited at 7～42 weeks of gestation with singleton pregnancy determined by ultrasound scan. QIAGEN DNA kits was used to extract fetal DNA from maternal plasma. Fluorescent quantitative PCR(FQ-PCR) were applied to determine the concentration of ?-actin and SRY gene in extracted DNA. Results Thirty-seven male fetus were all identified among the 58 pregnant women (100%) and no Y signal was detected in the other 21 pregnancies with female foetus. The median level of fetal DNA in those pregnancies with male foetus was 9.08 copies/ml (3.5～12.8) in early pregnancy,45.41 copies/ml (14.38～76.5) in mid-pregnancy and 300.95 copies/ml (84～840) in late pregnancy. Conclusions The concentration of fetal DNA increases with the progress of pregnancy. Maternal plasma may be valuable in noninvasive prenatal diagnosis.
