Effect of 60% oxygen exposure on expression of Bax and Bcl-X_L in lungs of newborn rats
- VernacularTitle:60%氧暴露对新生大鼠肺组织细胞凋亡相关基因表达的影响
- Author:
Anru WANG
;
Wei WANG
;
Xin LIU
- Publication Type:Journal Article
- Keywords:
Hyperoxia;
Bronchopulmonary dysplasia;
Apoptosis;
Gene, Bax;
Gene, Bcl-X_L
- From:
Chinese Journal of Perinatal Medicine
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Bax and Bcl-X_L expression in newborn rat with moderate hyperoxic exposure. Methods Hyperoxic lung injury model was established by exposure to 60% O_2 in the neonatal period of SD rats. Rats exposed to air were used as control groups, with 8 animals in each group on repeated experiments. The pathology of pulmonary tissues was detected by HE stain. Mean alveolar area and alveolar number per ?m~2 were applied to estimate the pathological effects of prolonged hyperoxia in neonatal rats. The expression of Bax and Bcl-X_L proteins in lung were detected by immunohistochemistry and the expressions of Bax and Bcl-X_L mRNA by RT-PCR. Results In hyperoxia groups, alveolar dysplasia appeared 4 days after hyperoxia, mean alveolar area increased and alveolar number per ?m~2 decreased from the 4th day. Bax and Bcl-X_L protein were mainly expressed on bronchiolar epithelial cells and vascular endothelial cells. Compared with control group, the expression of Bax increased from the 1st day after hyperoxia, Bax mRNA decreased from the 11th day (q=8.4802, P
