Antigen-loaded dendritic cells and CD40L triggers the killing effects of cytotoxic T lymphocytes on K562 cells in vitro
- VernacularTitle:肿瘤抗原联合CD40L致敏树突状细胞诱导CTLs杀伤K562细胞的实验研究
- Author:
Dongjun LIN
;
Zhigang FANG
;
Yongmei FU
;
Xudong LI
;
Renwei HUANG
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Frozen-thawed antigen;
CD40 ligand;
K562 cells;
T-lymphocytes,cytotoxic
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the anti-tumor effects of special cytotoxic T lymphocytes(CTLs)activated by dendritic cells(DCs)loaded with antigens and CD40L in vitro.METHODS:Peripheral blood mononuclear cells were isolated by Ficoll density gradient centrifugation from normal human heparinized blood.The adherent cells were cultured with granulocyte-macrophage colony stimulating factor(GM-CSF),interleukin-4(IL-4),alpha tumor necrosis factor(TNF-?),DCs were co-cultured with frozen-thawed antigen of K562 cells and CD40L,then triggered T cells into specific CTLs.RESULTS:Most suspended cells exhibited distinctive morphological features of DCs which expressed CD40 96%,CD86 97%,CD80 77%,CD1a 69%,and gained the powerful capacity to stimulate proliferation of allogenic lymphocytes.Under the effector∶target ratio of 20∶1,CTLs derived from cultures with DCs and frozen-thawed antigen of K562 cells were showed 71.3% cytotoxicities against K562 cells.CTLs derived from cultures with DCs loaded with frozen-thawed antigen and CD40L were showed 86.9% cytotoxicities against K562 cells.Cytotoxicities by CTLs derived from cultures with unloaded DCs against K562 cells were 37.6% and cytotoxicities by monocytes were 21.1%.Cytotoxicities by CTLs derived from experiment groups were stronger than control groups(P
