The suppressive effects of IFN-? on human gastric carcinoma cell line BGC-823
- VernacularTitle:IFN-?对人胃癌细胞株BGC-823生长的抑制作用(英文)
- Author:
Xin LIU
;
Yong WANG
;
Xingbin PAN
;
Heping WANG
;
Yueping ZHANG
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
BGC-823 cells;
Interferon-alpha
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the inhibitory effect of interferon-?(IFN-?)on the growth invasiveness and metastasis of human gastric carcinoma cell line BGC-823,and mechanism of its action.METHODS:We detected the influence of IFN-? on the proliferative ability of BGC-823 in cell culture system,the cell vitality with the MTT colorimetric assay,and the cell cycle with flow cytometer(FCM).The regulatory functions of IFN-? to the expression of E-cadherin and matrix metalloproteinase-2(MMP-2)in tumor cells were estimated by immunohistochemical analysis(S-P).The ultrastructural changes of the junction among the tumor cells were observed under electron microscope.RESULTS:IFN-? can significantly inhibit the growth of human gastric carcinoma cell line BGC-823 in a dose-dependent manner.When the concentration of IFN-? was ≥106 U/L,the cell proliferation can be effectively suppressed,the suppression rate was ≥12.2%,and the blockage appeared at the phase of G_1-S of the cell cycle.Under the induction of IFN-?,the expression level of the cell E-cadherin increased while the MMP-2 decreased.The changes on ultrastructure of the cells showed the increased adhesive junctions and the relative compact structure.CONCLUSION:IFN-? can suppress the growth of human gastric carcinoma cell line BGC-823 through its influence on cell cycle.IFN-? can regulate the expression of E-cadherin and MMP-2,make the cell junction closely,so that it has the potential on restricting the invasion and metastasis of gastric carcinoma cells.
