Effects of Huiyang shengji gao on proliferation of human fibroblasts derived from normal and chronic ulcer margin skin
- VernacularTitle:回阳生肌膏提取物对正常皮肤及慢性溃疡创缘成纤维细胞增殖的影响
- Author:
Ying ZHANG
;
Ping LI
;
Fang WANG
;
Xun SHENG
;
Yufeng JIANG
- Publication Type:Journal Article
- Keywords:
Fibroblasts;
Drugs,Chinese;
herbal;
Ulcer
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To compare the effects of alcohol and aqueous extracts from Huiyang shengji gao(HYSJG),a Chinese medicine,on proliferation of human fibroblasts derived from normal and ulcer margin skin.METHODS:Using explant culture techniques,we obtained populations of normal human fibroblast(nHFB)and ulcer marginal fibroblast(uHFB).Alarmar blue assay was used for observing cell proliferation,and flow cytometry for cell cycle analysis.RESULTS:After cultured with aqueous extracts and alcohol extracts of HYSJG for 24 hours,the proliferation rates of the two cell lines were both elevated.For alcohol extract,obvious proliferation-promoting concentrations on nHFB and uHFB were 0.027-0.425 mg/L and 0.027-0.213 mg/L,respectively(P
