Determination of Resveratrol in Serum and Organs of Mice by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定小鼠血清及脏器中白藜芦醇浓度
- Author:
Li WANG
;
Hong ZHOU
;
Sanqi ZHANG
;
Aidong WEN
;
Yi GU
;
Jiafang LIAN
;
Jingwen WANG
- Publication Type:Journal Article
- Keywords:
RP- HPLC;
Resveratrol;
Serum;
Tissue homogenates
- From:
China Pharmacy
2005;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To develop RP-HPLC method for the rapid determination of resveratrol in serum and tissue homogenates (in some organs) of mice.METHODS:HPLC determination was performed using Kromasil C18 column,the mobile phase consisted of methanol - water (48∶ 52) with a flow rate of 1ml/min,the column was under room temperature with its pressure at 12kPa,the detection wavelength was 303nm and the sample size was 20?l. RESULTS: The linear range for resveratrol was 0.25～25?g/ml, with minimal detectable drug concentration at 0.1?g/ml (S/N=3). The average absolute rec_overy of the samples was above 88% and the methodological recovery rates of which ranged from 96% to 100%,RSD were below 10%. CONCLUSIONS: The established determination method is economical, reliable, simple and rapid, and suitable for the blood concentration determination of resveratrol and the study of its distribution in animal body.
