The effect of cinobufacin combined with 5-FU on inhibiting proliferation and inducting apoptosis of human gastric carcinoma cells
- VernacularTitle:联合应用华蟾素与氟尿嘧啶对胃癌细胞增殖抑制及诱导凋亡的作用
- Author:
Hongbin HAN
;
Jiayong CHEN
;
Yong YUAN
;
Daoming LIANG
;
Yi ZHANG
- Publication Type:Journal Article
- Keywords:
Cinobufacin/ther use;
Fluorouracil/ther use;
Stomach Neoplasms/drug ther;
Tumor Cells, Cultured/drug ther;
Apoptosis
- From:
Chinese Journal of General Surgery
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of cinobufacin(cino) combined with fluorouracil(5-FU) on inhibiting proliferation and inducing apoptosis of human gastric carcinoma cells in vitro. Methods The experiment was divided into control group,cinobufacin group,5-FU group and cino+5-FU group. Cell morphological variation,cell inhibitory rate, cell cycle and ratio of apoptotic cell of human gastric carcinoma cell line BGC-823 were studied by cell culture, inverse microscopy, fluoroscopy, MTT assay and flow cytometry on different concentrations of cino and 5-FU. Results Cino could markably inhibit proliferation of human gastric carcinoma cells in time-and dose-dependent response. The cino+5-FU group inhibited the rate of proliferation of BGC-823 cells was significantly more than either cino or 5-FU alone group(P
