Human bone marrow mesenchymal stem cells differentiate toward endothelial lineagein vitro
- VernacularTitle:人骨髓间充质干细胞体外内皮细胞诱导分化的研究(英文)
- Author:
Xin GUO
;
Yulin LI
;
Xu HE
;
Wei LI
;
Yilei LI
;
Lihong ZHANG
;
Ruiguang YANG
;
Wei ZHAO
- Publication Type:Journal Article
- Keywords:
Bone marrow mesenchymal stem cells;
Endothelial cells;
Endothelial growth factors;
Fibronec-tins;
Collagen type I
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the cytological basis and differentiating conditions of human bone marrowmes-enchymal stem cells(hMSCs) differentiated into cells of the endothelial lineagein vitro.METHODS:hMSCs were isolatedby density gradient centrifugation and fractionated on a 1 073 g/L Percoll.The combination of VEGF165and various matrixproteins including fibronectin(FN) and typeⅠ collagen(Col) was used to induce hMSCsin vitro.Cells were character-ized by immunohistochemistry,cytochemistry,FACS and ultrastructure to identify and detect the differentiated populationand markers.RESULTS:hMSCs was positive for KDR.PAS reaction was positive and ultrastructure of hMSCs showedglycogen-pool in ectoplasm.Glycogen reducing or disappear suggested that stem cells have occurred differentiation.In-duction of hMSCs resulted in the increase of KDR,?1integrin and CD34.CONCLUSION:hMSCs were induced to atransit population(TP) that differentiated toward the endothelial progenitor cells(EPC),but not a really EPC.hMSCspedigree diagram of differentiation was hMSCs→TP→EPC→endothelial cells(ECs).
