Effect of protein kinase C signal pathway on resistin expression in 3T3-L1 adipocytes
- VernacularTitle:蛋白激酶C对3T3-L1脂肪细胞抵抗素表达的影响
- Author:
Zaigang YANG
;
Muxun ZHANG
;
Jianhua ZHANG
;
Hongwei WANG
- Publication Type:Journal Article
- Keywords:
Protein kinase C;
3T3-L1 adipocytes;
Resistin
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of protein kinase C on resistin expression in 3T3-L1 adipocytes.METHODS: The differentiated 3T3-L1 adipocytes were incubated with 50 nmol/L phorbol 12-myristate 13-acetate(PMA) or(5 ?mol/L) Ro-31-8220 for 24 h.Expression of resistin mRNA was detected by RT-PCR and expression of resistin protein was detected by Western blotting.RESULTS: Compared with control,PMA increased the expression of resistin mRNA and protein in 3T3-L1 adipocytes significantly(P
