Determination of Chlorogenic Acid and Baicalin in Xiaoer Qingrening Granula by HPLC
- VernacularTitle:高效液相色谱法测定小儿清热宁颗粒中绿原酸和黄芩苷的含量
- Author:
Quanmin FAN
;
Huijuan ZHOU
;
Dongyun SUN
- Publication Type:Journal Article
- Keywords:
HPLC;
Xiaoer qingrening granula;
Chlorogenic acid;
Baicalin;
Content
- From:
China Pharmacy
2005;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for the determination of chlorogenic acid and baicalin in xiaoer qingrening granula.METHODS:The determination was performed on Symmetry Shield RP C 18 ,the mobile phase of chlorogenic acid consisted of acetonitrile-0.4%phosphoric acid soluton(13∶87)and that of baicalin consisted of methanol-water-glacial acetic acid(50∶50∶1),the detection wavelengths of chlorogenic acid and baicalin were327nm and274nm,respectively,the flow rate was1.0ml/min,sample size was20?l and column temperature was25℃.RESULTS:The respective linear sample size range for chlorogenic acid and baicalin was0.1040?g～1.040?g(r=0.9998)and0.6240?g～6.240?g(r=0.9997),with average recoveries respectively at96.4%(RSD=1.4%)and98.0%(RSD=1.1%).CONCLUSIONS:The method is simple,accurate,reproducible and specific,and it can be used for the quality control of xiaoer qingrening granula.
